Journal: Global Implementation Research and Applications

Article Title: Measuring the Success of a Project ECHO Implementation: Results from an International e-Delphi Study

doi: 10.1007/s43477-022-00050-7

Figure Lengend Snippet: Phases of the e-Delphi study

Article Snippet: The Delphi technique originated at the RAND Corporation in the 1950s and has proven to be a popular method to identify and prioritise issues for decision-making, with well-documented and stepwise frameworks to investigate research questions (Okoli & Pawlowski, ; Schmidt, ).

Techniques:

Journal: Cancer Science

Article Title: TGF ‐β Inhibitor Potentiates Osimertinib‐Induced Anti‐Tumor Immunity in Egfr ‐Mutant Lung Cancer

doi: 10.1111/cas.70352

Figure Lengend Snippet: Enhancement of antitumor effect by combination therapy with osimertinib and nintedanib in vivo. (A) Representative images of TGF‐β immunohistochemical staining in Egfr ‐mutant lung tumors treated with osimertinib (15 mg/kg, 7 days/week) and vehicle for 4 days. Quantified with ImageJ (mean ± SEM, n = 12 fields/group). Scale bars: 100 μm. ****, p < 0.0001, Student's t ‐test. (B) Western blot analysis of TGF‐β in tumors treated with osimertinib and vehicle for 4 days. (C, D) Tumor growth (C; n = 6 tumors per group, 3 mice per group) and body weight (D; n = 3 mice per group) in Egfr ‐mutant lung cancer model treated with nintedanib (50 mg/kg, 7 days/week) and vehicle. Error bars: SEM. ns, not significant, Student's t ‐test. Representative of three independent experiments. (E, F) Tumor growth (E; n = 6 tumors per group, 3 mice per group) and body weight (F; n = 3 mice per group) in the model treated with osimertinib and osimertinib plus nintedanib for 14 days, followed by observation. Error bars: SEM. **, p < 0.01, Student's t ‐test. Representative of three independent experiments. (G, H) Cell proliferation assays in PC‐9 (G) or H1975 (H) cells treated with nintedanib, osimertinib, or their combination for 96 h. The data were the integrated results of three independent experiments with matching conditions. n = 9/group. Error bars: SEM. ns, not significant; ****, p < 0.0001, one‐way ANOVA with post hoc Tukey's test.

Article Snippet: PC‐9 (EGFR Ex19 del E746_A750) and NCI‐H1975 (EGFR L858R and T790M) cells were purchased from the European Collection of Authenticated Cell Cultures (Salisbury, UK) and the ATCC (Rockville, MD, USA), respectively.

Techniques: In Vivo, Immunohistochemical staining, Staining, Mutagenesis, Western Blot